7EOE

Crystal structure of CCDC25 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	193
Detector technology	PIXEL
Collection date	2020-10-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	124.192, 47.730, 83.024
Unit cell angles	90.00, 118.84, 90.00

Refinement procedure

Resolution	27.197 - 2.900
R-factor	0.2526
Rwork	0.251
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	de novo prediction
RMSD bond length	0.004
RMSD bond angle	0.634
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.138	0.333
Number of reflections	9735	820
<I/σ(I)>	11.5
Completeness [%]	97.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M CHES 9.5, 50% PEG 200