7ENR

Crystal structure of cas and anti-cas protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	H 3
Unit cell lengths	173.800, 173.800, 185.894
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.993 - 4.205
R-factor	0.2649
Rwork	0.260
R-free	0.30680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5czz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.350
High resolution limit [Å]	4.200	9.030	4.200
Rmerge	0.103	0.072	0.922
Rmeas	0.117	0.082	1.044
Rpim	0.056	0.039	0.486
Total number of observations	67938
Number of reflections	15197	1517	1501
<I/σ(I)>	5.7
Completeness [%]	100.0	99.8	100
Redundancy	4.5	4.4	4.6
CC(1/2)		0.988	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350, EG, Bicine