7ENI

Crystal structure of cas and anti-cas protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	87.852, 105.957, 238.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.237 - 2.632
R-factor	0.201
Rwork	0.200
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5czz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.710
High resolution limit [Å]	2.632	6.530	2.650
Rmerge	0.159	0.043	0.916
Rmeas	0.175	0.047	1.025
Rpim	0.073	0.019	0.449
Number of reflections	65529	4714	4242
<I/σ(I)>	5.6
Completeness [%]	98.3	99	96.9
Redundancy	5.3	5.8	4.6
CC(1/2)		0.998	0.298

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	Tris, Ammonium phosphate dibasic