7ENH

Crystal structure of cas and anti-cas protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 41 21 2
Unit cell lengths	68.564, 68.564, 118.697
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.482 - 2.097
R-factor	0.1835
Rwork	0.181
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5czz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.150
High resolution limit [Å]	2.097	5.180	2.100
Rmerge	0.097	0.045	0.713
Rmeas	0.101	0.047	0.747
Rpim	0.028	0.013	0.221
Number of reflections	29098	1302	1129
<I/σ(I)>	8
Completeness [%]	99.9	99.8	99.9
Redundancy	12.1	12.4	11.2
CC(1/2)		0.999	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	PEG 3350, sodium iodide