7EN4
Multi-state structure determination and dynamics analysis elucidate a new ubiquitin-recognition mechanism of yeast ubiquitin C-terminal hydrolase.
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 600 | |
| 2 | Bruker | AVANCE III | 600 | |
| 3 | Bruker | AVANCE III | 700 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 3D HNCA |
| 10 | 1 | 1 | 3D C(CO)NH |
| 11 | 1 | 1 | 3D H(CCO)NH |
| 12 | 1 | 3 | 3D H(CCO)NH |
| 13 | 1 | 3 | 3D C(CO)NH |
| 14 | 1 | 3 | 3D CCNH |
| 15 | 1 | 3 | 3D 1H-13C NOESY |
| 16 | 1 | 3 | 2D 1H-1H TOCSY |
| 17 | 1 | 3 | 3D 1H-15N NOESY |
| 18 | 1 | 3 | 3D TROSY-HNCO |
| 19 | 1 | 2 | heteronuclear 1H-15N NOE |
| 2 | 1 | 1 | 3D HN(CO)CA |
| 20 | 1 | 2 | T2 relaxation |
| 21 | 1 | 2 | T1 relaxation |
| 22 | 1 | 2 | T2 relaxation |
| 23 | 1 | 2 | T1 relaxation |
| 24 | 1 | 2 | heteronuclear 1H-15N NOE |
| 25 | 1 | 2 | 2D 1H-15N HSQC |
| 26 | 1 | 2 | 2D 1H-15N IPAP HSQC |
| 27 | 1 | 1 | 2D 1H-13C HSQC |
| 3 | 1 | 1 | 3D CBCA(CO)NH |
| 4 | 1 | 1 | 3D CBCANH |
| 5 | 1 | 1 | 3D HBHA(CO)NH |
| 6 | 1 | 1 | 3D HSQC-NOESY-HSQC |
| 7 | 1 | 1 | 3D 1H-15N NOESY |
| 8 | 1 | 1 | 3D 1H-13C NOESY |
| 9 | 1 | 1 | 3D HCCH-TOCSY |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6 | AMBIENT | 303 |
Conformers
| Conformers Calculated Total Number | 20 |
| Conformers Submitted Total Number | 20 |
