7EL6
Structure of SMCR8 bound FEM1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97853 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 83.306, 91.497, 112.148 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.383 - 2.802 |
R-factor | 0.2091 |
Rwork | 0.203 |
R-free | 0.26580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lbf |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.383 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 20988 | 1998 |
<I/σ(I)> | 49.5 | |
Completeness [%] | 100.0 | |
Redundancy | 12.8 | |
CC(1/2) | 1.000 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Tris pH7.3 4% PEG8000 |