7EL5

Crystal structure of Sizzled protein from Xenopus Laevis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	63.880, 77.480, 107.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 1.950
R-factor	0.207
Rwork	0.205
R-free	0.24300
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.910	2.020
High resolution limit [Å]	1.950	1.950
Number of reflections	74377	5413
<I/σ(I)>	15.58
Completeness [%]	98.9	98.3
Redundancy	13.7
CC(1/2)	0.830	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.2 M NH4Ac, 18 % PEG 3350