7EKZ
Structural and functional insights into Hydra Actinoporin-Like Toxin 1 (HALT-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-29 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.100, 49.590, 77.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.550 - 1.430 |
| R-factor | 0.1658 |
| Rwork | 0.163 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gwy |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.105 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.550 | 1.450 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.147 | 0.419 |
| Rmeas | 0.163 | 0.506 |
| Rpim | 0.068 | 0.276 |
| Number of reflections | 33402 | 1577 |
| <I/σ(I)> | 5.2 | 2 |
| Completeness [%] | 98.2 | 95.2 |
| Redundancy | 4.8 | 3 |
| CC(1/2) | 0.983 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 0.1 M sodium formate, 22% w/v polyethylene glycol 3550 |
