7EKW
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (D535N) in complex with maltotetrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97939 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.250, 198.940, 134.590 |
Unit cell angles | 90.00, 104.87, 90.00 |
Refinement procedure
Resolution | 25.260 - 3.100 |
R-factor | 0.2493 |
Rwork | 0.248 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d0f |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.750 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 73585 | 10713 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 3.7 | |
CC(1/2) | 0.991 | 0.564 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride |