7EKU
Crystal Structure of the Candida Glabrata Glycogen Debranching Enzyme (W958A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 160.221, 200.651, 259.976 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.800 - 3.100 |
R-factor | 0.2326 |
Rwork | 0.231 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5d06 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.800 | 46.800 | 3.180 |
High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
Rmerge | 0.100 | 0.036 | 0.950 |
Rmeas | 0.107 | 0.039 | 1.020 |
Total number of observations | 562556 | ||
Number of reflections | 75998 | 912 | 5569 |
<I/σ(I)> | 15.39 | 36.57 | 1.95 |
Completeness [%] | 99.9 | 94.8 | 100 |
Redundancy | 7.402 | 6.127 | 7.555 |
CC(1/2) | 0.998 | 0.997 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 5000 MME,0.1 M Tris pH7.0,5% Tasimate pH7.0, 3.5mM TCEP hydrochiloride |