7EKR

The crystal structure of Orange carotenoid binding protein 1 (OCP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-14
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	82.944, 82.944, 197.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.410 - 2.170
R-factor	0.1847
Rwork	0.182
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pq1
RMSD bond length	0.007
RMSD bond angle	1.737
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.410	2.250
High resolution limit [Å]	2.170	2.170
Rmerge	0.250	0.750
Number of reflections	37054	3399
<I/σ(I)>	38.91
Completeness [%]	99.3	93.2
Redundancy	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Sodium Formation and 22% PEG3350