7EKN

Crystal structure of AF10-ipep complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9788
Spacegroup name	P 1
Unit cell lengths	42.626, 46.389, 49.170
Unit cell angles	76.67, 67.70, 74.67

Refinement procedure

Resolution	45.010 - 2.140
R-factor	0.1936
Rwork	0.186
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7edp
RMSD bond length	0.007
RMSD bond angle	0.747
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.310
High resolution limit [Å]	2.140	2.140
Rmerge	0.129
Rmeas	0.182
Rpim	0.129
Number of reflections	17862	30000
<I/σ(I)>	10.2
Completeness [%]	97.5
Redundancy	3.8
CC(1/2)		0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG4000, Tris8.5, Sodium acetate