7EKG

Structure of SARS-CoV-2 Beta variant spike receptor-binding domain complexed with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	105.150, 105.150, 230.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.910 - 2.630
R-factor	0.2156
Rwork	0.214
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.004
RMSD bond angle	0.621
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.010	2.700
High resolution limit [Å]	2.630	2.630
Number of reflections	39380	39271
<I/σ(I)>	21.9
Completeness [%]	99.0
Redundancy	21.8
CC(1/2)	1.000	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MES, pH 6.5, 12% w/v PEG 20000