7EKD
Crystal structure of gibberellin 3-oxidase 2 (GA3ox2) in rice
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.838, 45.594, 68.538 |
| Unit cell angles | 90.00, 102.26, 90.00 |
Refinement procedure
| Resolution | 37.690 - 1.899 |
| R-factor | 0.1655 |
| Rwork | 0.163 |
| R-free | 0.21220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ku3 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.899 | 1.900 |
| Rmerge | 0.047 | 0.221 |
| Number of reflections | 27238 | 1267 |
| <I/σ(I)> | 38.4 | 7.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M ammonium sulfate, 0.1 M Sodium HEPES (pH 7.0) and 20% (w/v) PEG4000 |
