7EKC

Structure of SARS-CoV-2 Gamma variant spike receptor-binding domain complexed with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	103.354, 103.354, 229.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.800
R-factor	0.2146
Rwork	0.214
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lzg
RMSD bond length	0.002
RMSD bond angle	0.477
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	31516	29219
<I/σ(I)>	17.884
Completeness [%]	99.9
Redundancy	11.3
CC(1/2)	0.997	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M MES, pH 6.5, 12% w/v PEG 20000