7EK8
Crystal structure of apo streptavidin at ambient temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE MFX |
Synchrotron site | SLAC LCLS |
Beamline | MFX |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2020-08-16 |
Detector | SLAC ePix10k 2M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.400, 87.700, 58.900 |
Unit cell angles | 90.00, 98.90, 90.00 |
Refinement procedure
Resolution | 48.488 - 1.700 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.22420 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | CrystFEL |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_3318) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.490 | 48.490 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 52270 | |
<I/σ(I)> | 7.66 | |
Completeness [%] | 100.0 | |
Redundancy | 2 | |
CC(1/2) | 0.960 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Pact PremierTM 100 mM MMT buffer pH 6.0 and 25 % w/v PEG 1500 |