7EK5

prawn ferritin to coordinate with heavy metal ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 63 2 2
Unit cell lengths	155.613, 155.613, 162.729
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.020 - 3.000
R-factor	0.1831
Rwork	0.181
R-free	0.22350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.020	6.440
High resolution limit [Å]	3.000	3.000
Number of reflections	23717	23717
<I/σ(I)>	8.7
Completeness [%]	99.6
Redundancy	10.4
CC(1/2)	0.990	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M Sodium acetate trihydrate pH 4.6, 2.0 M Sodium chloride