7EK4

prawn ferritin to coordinate with heavy metal ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	I 4
Unit cell lengths	125.372, 125.372, 176.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.550 - 2.300
R-factor	0.197
Rwork	0.195
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.550	4.950
High resolution limit [Å]	2.300	2.300
Rpim	0.072
Number of reflections	60408	60408
<I/σ(I)>	5.8
Completeness [%]	99.8
Redundancy	3.4
CC(1/2)	0.982	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M HEPES pH 7.5, 2.0 M Ammonium format