7EJY

Complex Structure of antibody BD-503 and RBD of COVID-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	80.432, 144.083, 146.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 - 3.040
R-factor	0.2184
Rwork	0.214
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bwj
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	3.149
High resolution limit [Å]	3.040	3.040
Rmerge		0.876
Rmeas	0.275	0.920
Rpim	0.077	0.272
Number of reflections	15571	934
<I/σ(I)>	10.61	2
Completeness [%]	92.7
Redundancy	12.5
CC(1/2)	0.971	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15M Ammonium sulfate, 0.1M Tris, pH 8.0, 15% (w/v) PEG 400