7EJS

Structure of ERH-2 bound to PICS-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	44.825, 52.528, 125.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.264 - 2.387
R-factor	0.2046
Rwork	0.201
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wz7
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.380	2.400
Number of reflections	11991	1679
<I/σ(I)>	24
Completeness [%]	100.0
Redundancy	11.6
CC(1/2)	0.996	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% v/v 2-propanol, 0.1M sodium citrate tribasic dihydrate pH 5.0, 10% PEG 10000