7EJM
Complex Structure of HLA-A*2402 with the Peptide from HCoV(CoV-229E) spike protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-01-28 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.946, 85.586, 91.091 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.950 - 1.710 |
R-factor | 0.1929 |
Rwork | 0.192 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f7m |
RMSD bond length | 0.007 |
RMSD bond angle | 0.826 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.950 | 1.770 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.096 | |
Number of reflections | 111576 | 11121 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 100.0 | |
Redundancy | 7.7 | |
CC(1/2) | 0.999 | 0.492 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Na citrate, PEG 3350 |