7EIV

heterotetrameric glycyl-tRNA synthetase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	F 2 2 2
Unit cell lengths	207.371, 253.945, 270.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.580 - 2.680
R-factor	0.2293
Rwork	0.228
R-free	0.24870
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.210
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.780
High resolution limit [Å]	2.680	5.770	2.680
Rmerge	0.084	0.064	0.602
Rmeas	0.087	0.066	0.627
Rpim	0.024	0.019	0.173
Total number of observations	1340079
Number of reflections	99458	10198	9862
<I/σ(I)>	9.4
Completeness [%]	100.0	99.7	100
Redundancy	13.5	12.8	13.1
CC(1/2)		0.998	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	291	0.4 M magnesium acetate, 0.1 M HEPES pH 6.8, 2% (v/v) PEG 3350, 2% (v/v) PEG 5000 MME, 2% (v/v) PEG 4000, 2% (v/v) PEG 2000 and 10% ethylene glycol