7EIO

Crystal Structure of Mei2 RRM3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	74.546, 74.546, 68.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.356 - 1.895
R-factor	0.1913
Rwork	0.189
R-free	0.22530
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.930
High resolution limit [Å]	1.895	1.900
Rmerge	0.074	1.002
Number of reflections	29356	1481
<I/σ(I)>	35.76
Completeness [%]	99.9
Redundancy	13.1
CC(1/2)	1.000	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Ammonium Sulfate, 0.1M Bis-Tris, PH 5.5, 25% PEG3350