7EIH

Ancestral L-Lys oxidase (ligand free form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	89.441, 80.462, 173.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 2.200
Rwork	0.214
R-free	0.25520
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.310
High resolution limit [Å]	2.200	2.200
Rmerge	0.106	0.846
Number of reflections	63996	9129
<I/σ(I)>	18.9
Completeness [%]	98.8
Redundancy	13.5
CC(1/2)	0.999	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	12.5% (w/v) PEG3350, 0.1 M HEPES-NaOH (pH 7.5) and 0.2 M ammonium sulfate