7EIF
Crystal structure of GAS41 YEATS domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.595, 52.834, 57.864 |
| Unit cell angles | 90.00, 116.47, 90.00 |
Refinement procedure
| Resolution | 32.960 - 1.580 |
| R-factor | 0.14481 |
| Rwork | 0.144 |
| R-free | 0.15812 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tmp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.575 | 1.575 |
| Number of reflections | 27444 | 1383 |
| <I/σ(I)> | 29.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-Tris buffer (pH 5.9) containing 3.3 M NaCl |
