7EIE
Crystal structure of YEATS2 YEATS domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-02 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.448, 50.840, 82.629 |
Unit cell angles | 90.00, 100.22, 90.00 |
Refinement procedure
Resolution | 44.550 - 1.670 |
R-factor | 0.17487 |
Rwork | 0.174 |
R-free | 0.20190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tmp |
RMSD bond length | 0.003 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Number of reflections | 43069 | 2170 |
<I/σ(I)> | 11.6 | |
Completeness [%] | 100.0 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl buffer (pH 8.5) containing 2 M NaCl |