7EIA
RNA kink-turn motif with pyrrolo cytosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 69.056, 77.575, 81.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.510 - 3.000 |
| R-factor | 0.2073 |
| Rwork | 0.200 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c40 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.510 | 43.510 | 3.100 |
| High resolution limit [Å] | 3.000 | 5.000 | 3.000 |
| Rmerge | 0.040 | 0.024 | 0.383 |
| Rmeas | 0.047 | 0.028 | 0.449 |
| Total number of observations | 29217 | ||
| Number of reflections | 8773 | 1895 | 792 |
| <I/σ(I)> | 18.3 | 37.63 | 2.68 |
| Completeness [%] | 95.7 | 90.5 | 95.8 |
| Redundancy | 3.33 | 3.225 | 3.391 |
| CC(1/2) | 0.999 | 0.999 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium cacodylate, MPD, spermine tetrahydrochloride, potassium chloride |
