7EI7

RNA kink-turn motif with 2-aminopurine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.60496
Spacegroup name	P 42 21 2
Unit cell lengths	75.934, 75.934, 41.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.970 - 3.200
R-factor	0.251
Rwork	0.245
R-free	0.30450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c40
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.970	37.970	3.280
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.066	0.028	0.448
Rmeas	0.068	0.029	0.458
Total number of observations	51123
Number of reflections	2201	32	150
<I/σ(I)>	39.55	86.6	8.76
Completeness [%]	100.0	97	100
Redundancy	23.227	16.219	24.613
CC(1/2)	1.000	1.000	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium cacodylate, hexammine cobalt, MPD, strontium chloride