7EI6
GNRA tetraloop receptor motif composed of RNA and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.10611 |
Spacegroup name | H 3 |
Unit cell lengths | 82.928, 82.928, 71.245 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.910 - 2.900 |
R-factor | 0.1686 |
Rwork | 0.165 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnj |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.910 | 31.910 | 2.980 |
High resolution limit [Å] | 2.900 | 12.980 | 2.900 |
Rmerge | 0.068 | 0.035 | 0.273 |
Rmeas | 0.086 | 0.045 | 0.341 |
Total number of observations | 20875 | ||
Number of reflections | 7926 | 83 | 619 |
<I/σ(I)> | 11.1 | 19.07 | 4.12 |
Completeness [%] | 97.8 | 88.3 | 99.5 |
Redundancy | 2.634 | 2.614 | 2.64 |
CC(1/2) | 0.995 | 0.998 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Sodium malonate pH7.0, HEPES pH7.0, Jeffamine ED-2001 pH7.0 |