7EI5

GNRA tetraloop receptor motif composed of RNA and DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-18
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	83.220, 83.220, 67.164
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.440 - 2.900
R-factor	0.1748
Rwork	0.172
R-free	0.19920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fnj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.440	30.440	2.970
High resolution limit [Å]	2.900	12.960	2.900
Rmerge	0.063	0.037	0.286
Rmeas	0.079	0.049	0.366
Total number of observations	20349
Number of reflections	7623	75	568
<I/σ(I)>	13.75	29.37	3.3
Completeness [%]	98.8	93.8	95
Redundancy	2.669	2.533	2.431
CC(1/2)	0.995	0.998	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium cacodylate, MPD, hexammine cobalt, strontium chloride