7EI1
Structure of Pyrococcus furiosus Cas1Cas2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.763, 180.473, 338.847 |
| Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
| Resolution | 36.530 - 3.900 |
| R-factor | 0.281 |
| Rwork | 0.281 |
| R-free | 0.32810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wj0 4tn0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.765 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rpim | 0.063 | |
| Number of reflections | 107906 | 5377 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.995 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% w/v polyethylene glycol 6000, 6% v/v ethylene glycol, 0.1M citric acid pH 3.5 |
