7EHU

Chitin oligosaccharide binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-19
Detector	RAYONIX MX-225
Wavelength(s)	0.85
Spacegroup name	P 1 21 1
Unit cell lengths	50.241, 69.616, 56.195
Unit cell angles	90.00, 108.37, 90.00

Refinement procedure

Resolution	29.150 - 1.200
R-factor	0.1417
Rwork	0.141
R-free	0.16360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eho
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Rmeas	0.078	1.279
Rpim	0.029	0.474
Number of reflections	114172	5714
<I/σ(I)>	41.6
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)		0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG3350, MES