7EHT
Levansucrase from Brenneria sp. EniD 312
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-08 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 97.974, 97.974, 214.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 84.800 - 1.450 |
Rwork | 0.172 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d47 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.800 | 1.510 |
High resolution limit [Å] | 1.450 | 1.450 |
Rpim | 0.028 | 0.486 |
Number of reflections | 3547784 | 91324 |
<I/σ(I)> | 22.2 | |
Completeness [%] | 90.5 | |
Redundancy | 38.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammounium sulfate, PEG 400 |