7EHS
Levansucrase from Brenneria sp. EniD 312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 98.314, 98.314, 216.475 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 85.140 - 1.600 |
| R-factor | 0.1584 |
| Rwork | 0.157 |
| R-free | 0.18090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d47 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.725 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.150 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rpim | 0.025 | 0.456 |
| Number of reflections | 80004 | 14170 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 94.6 | 82.3 |
| Redundancy | 39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammonium sulfate, PEG 400 |
