7EHR
Levansucrase from Brenneria sp. EniD 312 at 1.33 angstroms resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 98.000, 98.000, 213.539 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 84.800 - 1.330 |
Rwork | 0.164 |
R-free | 0.17600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d47 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.685 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.870 | 1.400 |
High resolution limit [Å] | 1.330 | 1.330 |
Rpim | 0.019 | 0.012 |
Number of reflections | 138038 | 6204 |
<I/σ(I)> | 20.1 | |
Completeness [%] | 90.3 | 34.5 |
Redundancy | 36.8 | 16.1 |
CC(1/2) | 0.999 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | Ammonium sulfate, PEG 400 |