7EHQ

Chitin oligosaccharide binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-13
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	136.517, 68.725, 56.752
Unit cell angles	90.00, 101.62, 90.00

Refinement procedure

Resolution	27.890 - 1.700
R-factor	0.1946
Rwork	0.193
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eho
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmeas	0.079	1.288
Rpim	0.031	0.492
Number of reflections	55696	2800
<I/σ(I)>	54.8	2.2
Completeness [%]	98.0
Redundancy	6.5
CC(1/2)		0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	PEG3350, MES