7EHG
Crystal Structure of MiXBM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.237, 66.145, 85.669 |
| Unit cell angles | 84.15, 89.30, 89.23 |
Refinement procedure
| Resolution | 27.420 - 1.950 |
| R-factor | 0.1708 |
| Rwork | 0.170 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f2p |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.961 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.039 | 0.064 |
| Rpim | 0.023 | 0.040 |
| Number of reflections | 87791 | 4433 |
| <I/σ(I)> | 33.7 | 20 |
| Completeness [%] | 96.8 | 96.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350 |
