7EHG

Crystal Structure of MiXBM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	56.237, 66.145, 85.669
Unit cell angles	84.15, 89.30, 89.23

Refinement procedure

Resolution	27.420 - 1.950
R-factor	0.1708
Rwork	0.170
R-free	0.19980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f2p
RMSD bond length	0.007
RMSD bond angle	0.961
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.039	0.064
Rpim	0.023	0.040
Number of reflections	87791	4433
<I/σ(I)>	33.7	20
Completeness [%]	96.8	96.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350