7EHA
Crystal structure of the flagellar hook cap from Salmonella enterica serovar Typhimurium
Replaces: 6IEEExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 40 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 141.283, 141.283, 153.487 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.200 - 3.300 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28400 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.780 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.109 | 0.401 |
| Number of reflections | 27142 | 3930 |
| <I/σ(I)> | 19 | 4.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 277 | PEG 2000, sodium citrate, 2,3-butanediol, glycerol |
