7EH9
Crystal structure of the flagellar hook cap fragment from Salmonella enterica serovar Typhimurium
Replaces: 6IEFExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 75.985, 104.323, 43.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.570 - 2.200 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.24800 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.028 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.700 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.081 | 0.272 |
Number of reflections | 18336 | 2615 |
<I/σ(I)> | 14.6 | 5.9 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG 3350, ammonium citrate dibasic |