7EH3
Structure of Nanobody Nb23 in solution using NMR spectroscopy
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE III | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 4 | 1 | 2D 1H-15N HSQC |
| 10 | 4 | 1 | 3D HN(CA)CO |
| 11 | 6 | 3 | 2D 1H-1H TOCSY |
| 12 | 5 | 2 | 3D 1H-15N NOESY |
| 13 | 5 | 5 | 2D HBCBCGCDHD |
| 14 | 5 | 5 | 2D HBCBCGCDCEHE |
| 15 | 7 | 4 | 2D 1H-1H NOESY |
| 16 | 5 | 5 | 3D 1H-13C NOESY aliphatic |
| 17 | 5 | 5 | 3D 1H-13C NOESY aromatic |
| 18 | 5 | 2 | 2D 1H-13C HSQC |
| 19 | 5 | 5 | 2D 1H-15N HSQC |
| 2 | 5 | 2 | 2D 1H-15N TROSY |
| 3 | 4 | 1 | 3D CBCANH |
| 4 | 4 | 1 | 3D CBCA(CO)NH |
| 5 | 4 | 1 | 3D HNCA |
| 6 | 4 | 1 | 3D C(CO)NH |
| 7 | 5 | 2 | 3D H(CCO)NH |
| 8 | 4 | 1 | 3D HBHA(CO)NH |
| 9 | 4 | 1 | 3D HNCO |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 4 | 6.95 | 1 | 298 |
| 5 | 6.95 | 1 | 298 |
| 6 | 6.95 | 1 | 298 |
| 7 | 6.95 | 1 | 298 |
Conformers
| Conformers Calculated Total Number | 20 |
| Conformers Submitted Total Number | 10 |
