7EGL
Bicarbonate transporter complex SbtA-SbtB bound to HCO3-
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9798 |
Spacegroup name | H 3 2 |
Unit cell lengths | 107.239, 107.239, 352.219 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.774 - 3.200 |
R-factor | 0.2189 |
Rwork | 0.213 |
R-free | 0.26950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7egk |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 8.670 | 3.260 |
High resolution limit [Å] | 3.200 | 6.890 | 3.200 |
Rmerge | 0.162 | 0.049 | 1.553 |
Rmeas | 0.171 | 0.052 | 1.642 |
Rpim | 0.054 | 0.016 | 0.529 |
Number of reflections | 15727 | 694 | 676 |
<I/σ(I)> | 3.9 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 9.6 | 9.8 | 9.3 |
CC(1/2) | 0.999 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.05M magnesium chloride, 0.1M glycine, 22% PEG 400 |