7EFY
Crystal structure of retroviral protease-like domain of Ddi1 from Cryptosporidium hominis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9918 |
Spacegroup name | H 3 2 |
Unit cell lengths | 81.559, 81.559, 142.181 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.439 - 2.800 |
Rwork | 0.184 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d66 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.497 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.260 | 2.800 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 4686 | 668 |
<I/σ(I)> | 11.1 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6 | 291 | 0.1 M MES-sodium hydroxide (pH 6.0), 30% polyacrylate sodium salt and 10% ethanol |