7EFV
Crystal structure of octameric state of C-phycocyanin from Thermoleptolyngbya sp. O-77
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 229.950, 229.950, 229.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.030 - 2.770 |
R-factor | 0.1991 |
Rwork | 0.196 |
R-free | 0.23750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i7y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.816 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.030 | 2.930 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmeas | 0.378 | 4.200 |
Number of reflections | 26620 | 4259 |
<I/σ(I)> | 11.39 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 61.6 | 61.7 |
CC(1/2) | 0.998 | 0.557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.6 M sodium chloride, 8% polyethylene glycol 6,000, 20% glycero |