7EFH
RNA kink-turn motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 51.434, 51.434, 44.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.720 - 2.700 |
| R-factor | 0.2307 |
| Rwork | 0.229 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cs1 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.720 | 25.720 | 2.780 |
| High resolution limit [Å] | 2.700 | 11.450 | 2.700 |
| Rmerge | 0.074 | 0.029 | 0.358 |
| Rmeas | 0.081 | 0.034 | 0.394 |
| Number of reflections | 1843 | 32 | 139 |
| <I/σ(I)> | 20.23 | 37.34 | 5.08 |
| Completeness [%] | 99.5 | 86.5 | 97.9 |
| Redundancy | 5.929 | 4.188 | 5.676 |
| CC(1/2) | 0.998 | 0.999 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium cacodylate, MPD, spermine tetrahydrochloride, magnesium chloride |
