7EFA
Crystal structure of the complex between the C-terminal domain of mouse MUTYH and human PCNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 |
| Unit cell lengths | 87.780, 87.780, 124.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.400 - 2.700 |
| R-factor | 0.2373 |
| Rwork | 0.235 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ul1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.498 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.043 | 1.190 |
| Number of reflections | 14970 | 11997 |
| <I/σ(I)> | 29.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG8000, imidazole |
