7EF2
Crystal structure of maize SHH2 SAWADEE domain in complex with an H3K9me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.092, 68.254, 80.811 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.669 - 2.000 |
| R-factor | 0.2162 |
| Rwork | 0.215 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eez |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.085 | 0.047 | 0.519 |
| Rmeas | 0.092 | 0.051 | 0.565 |
| Rpim | 0.035 | 0.020 | 0.219 |
| Total number of observations | 175521 | ||
| Number of reflections | 25032 | 2648 | 2319 |
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.0 | 98 | 93.5 |
| Redundancy | 7 | 6.4 | 6.4 |
| CC(1/2) | 0.998 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES, pH7.5, 70% MPD |
