7EF1
crystal structure of maize SHH2 SAWADEE domain in complex with and H3K9me1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.821, 68.563, 80.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.863 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eez |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.877 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.102 | 0.034 | 0.757 |
| Rmeas | 0.116 | 0.040 | 0.869 |
| Rpim | 0.055 | 0.019 | 0.421 |
| Total number of observations | 116568 | ||
| Number of reflections | 29252 | 3105 | 2883 |
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 99.6 | 99.2 | 99.5 |
| Redundancy | 4 | 3.9 | 3.9 |
| CC(1/2) | 0.998 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES, pH7.5, 70% MPD |
