7EEZ
crystal structure of maize SHH2 SAWADEE domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 47.262, 47.262, 137.568 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.953 - 1.902 |
| R-factor | 0.2215 |
| Rwork | 0.221 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iur |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.079 | 0.045 | 0.720 |
| Rmeas | 0.082 | 0.046 | 0.744 |
| Rpim | 0.021 | 0.012 | 0.185 |
| Total number of observations | 201808 | ||
| Number of reflections | 13044 | 1474 | 1275 |
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 99.9 | 99.5 | 100 |
| Redundancy | 15.5 | 13.8 | 15 |
| CC(1/2) | 0.999 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH 6.5, 30% PEG 300 |
