7EEO
Bulged-G motif composed of RNA, DNA and 2'-O-methyl RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.60496 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 24.776, 116.432, 27.904 |
Unit cell angles | 90.00, 107.22, 90.00 |
Refinement procedure
Resolution | 29.108 - 2.701 |
R-factor | 0.2128 |
Rwork | 0.207 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q9a |
RMSD bond length | 0.006 |
RMSD bond angle | 1.531 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.108 | 29.108 | 2.770 |
High resolution limit [Å] | 2.700 | 12.080 | 2.700 |
Rmerge | 0.054 | 0.032 | 0.267 |
Rmeas | 0.077 | 0.045 | 0.373 |
Number of reflections | 7521 | 75 | 555 |
<I/σ(I)> | 8.33 | 14.56 | 2.36 |
Completeness [%] | 90.3 | 78.1 | 85.3 |
Redundancy | 1.565 | 1.52 | 1.564 |
CC(1/2) | 0.994 | 0.993 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium chloride, MPD, hexammine cobalt, lithium chloride |