7EEM
RNA bulged-G motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.10567 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 25.132, 53.316, 105.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.658 - 2.593 |
| R-factor | 0.1861 |
| Rwork | 0.180 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q9a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.658 | 26.658 | 2.660 |
| High resolution limit [Å] | 2.590 | 11.600 | 2.590 |
| Rmerge | 0.089 | 0.031 | 0.374 |
| Rmeas | 0.106 | 0.038 | 0.448 |
| Number of reflections | 4239 | 47 | 330 |
| <I/σ(I)> | 10.87 | 18.4 | 3.97 |
| Completeness [%] | 99.1 | 97.9 | 96.5 |
| Redundancy | 3.397 | 3.362 | 3.194 |
| CC(1/2) | 0.992 | 0.998 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium cacodylate, MPD, hexammine cobalt, potassium chloride |
